Table 2.
Cold-active recombinant microbial enzymes and their potential applications in food industry
Cold-active enzymes | Sample source | Source microorganisms | Cloning/expression host | Expression vector | Potential/recommended applications | References |
---|---|---|---|---|---|---|
Xylanase | Sea water (China) | Zunongwangia profunda | Escherichia coli BL21, E. coli DH5α | pGEX-6p-1 | Food industry for processing of sea and saline foods | Liu et al. (2014) |
NS | Saccharophagusdegradans 2–40 | E. coli DH5α, E. coli BL21 | pET21a | Baking industry | Ko et al. (2016) | |
NS | Pseudoalteromons haloplanktis TAH3A, Flavobacterium sp. MSY-2 | E. coli BL21 | pET22b-XFH-His6, pET22b-Xyn8-His6 | Baking industry | Dornez et al. (2011) | |
NS | Glaciecola mesophile | E. coli BL21 | pET22b | Baking industry | Zheng et al. (2011) | |
NS | Flavobacterium johnsoniae | E. coli JM109, E. coli DH5α, E. coli BL21 | pSCH602, pSCH643 | Food and feed industry | Chen et al. (2013) | |
β-Galactosidase | Calcium carbonate (Ikka columns, South-West Greenland) | Alkalilactibacillusikkense | E. coli TOP10 | pUC18dLacZ | -Do- | Schmidt and Stougaard (2010) |
Soil (Antarctica) | Arthrobacter sp. 20B | E. coli TOP10F’ | NS | -Do- | Białkowska et al. (2009) | |
Soil (Antarctica) | Paracoccus sp. 32d | E. coli LMG 194 | pBAD/Myc-His A | -Do- | Wierzbicka-Woś et al. (2011) | |
Deep sea water (Mariana Trench) | Alteromonas sp. ML52 | E. coli BL21 | pET-gal | -Do- | Sun et al. (2018) | |
Alimentary tract (krill Thyssanoessamacrura) (Southern Shetlands) | Pseudoalteromonas sp. 22b | E. coli ER2566 | pETbeta22b | -Do- | Cieśliński et al. (2005) | |
Frozen soil (China) | Rahnella sp. R3 | E. coli BL21 | pCold I | -Do- | Fan et al. (2015) | |
α-amylase | Surface sea water (China) | Zunongwangia profunda (MCCC 1A01486) | E. coli DH5α, E. coli BL21 | pGEX-6P-1 | Baking industry | Qin et al. (2014) |
Deep sea sediment (China) | Bacillus sp. dsh19‑1 | E. coli JM109, E. coli BL21 | pColdI | Starch hydrolysis | Dou et al. (2018) | |
Sea ice (Antarctica) | Pseudoalteromonas sp. M175 | E. coli BL21 | pET-amy175 | Food industry | Wang et al. (2018a) | |
Soil (Tehran) | Exiguobacterium sp. SH3 | E. coli DH5α, E. coli BL21 | pEamy | Food industry | Mojallali et al. (2014), Emampour et al. (2015) | |
Soil (King George Island, Antarctica) | Geomycespannorum | E. coli DH5α, Aspergillus oryzae | pBC-hygro | Food industry | Krishnan et al. (2011), Mao et al. (2015) | |
NS | Bifidobacterium longum | E. coli MC1061 | NS | In food processing industry for production of slowly digestible starch | Lee et al. (2016) | |
β-Glucosidase | Konjac field (South Korea) | Paenibacillusxylanilyticus KJ-03 | E. coli JM109, E. coli BL21 | pColdI | Food processing industry | Park et al. (2013) |
Pullulanase | Soil (Tehran) | Exiguobacterium sp. SH3 | E. coli BL21 | pET-26b (+) | Cold hydrolysis of starch and in sugar syrup production | Mojallali et al. (2014), Rajaei et al. (2015) |
Soil (China) | PaenibacilluspolymyxaNws-pp2 | E. coli DH5α, E. coli BL21 | pET-28a, pET-32a and pET-42a | Food processing industry | Wei et al. (2015) | |
Hot-spring (Sikkim, India) | Hot-spring metagenome | E. coli BL21 | pET28a(+) | Production of resistant starch | Thakur et al. (2021b) | |
Waste water (Europe) | Bacillus methanolicus PB1 | E. coli DH5α, E. coli BL21 | pET-28a | Cold hydrolysis of starch | Heggeset et al. (2012), Zhang et al. (2020) | |
Chitinase | Sea sediment (China) | Pseudoalteromonas sp. DL-6 | E. coli BL21 | pET28a | Biocontrol of microbial spoilage of cold-stored foods | Wang et al. (2014a; b) |
Sea ice (Antarctica) | Glaciozyma antarctica PI12 | E. coli JM109, Pichia pastoris | pPICZα A | Biocontrol of microbial spoilage of cold-stored foods | Ramli et al. (2013) | |
Lipase/esterase | Deep sea water (Indian ocean) | Aeromicrobium sp. SCSIO 25071 | E. coli DH5α, E. coli Rosetta (DE3) | pET28a | As food additives | Su et al. (2016) |
Marine sediment (Arctic) | Halocynthiibacter arcticus | E. coli BL21 | pET-21a | Production of flavored compounds | Le et al. (2020) | |
NS | Streptomyces coelicolor A3(2) | E. coli BL21 (DE3) | pET16b | Production of flavored compounds | Brault et al. (2012) | |
NS | Malassezia globose | E. coli Top10, P. pastoris X-33 | pGAPZαA | Oil modification and food processing industry | Xu et al. (2015) | |
Car service contaminated soil (Malaysia) | Staphylococcus epidermidis AT2 | E. coli Tuner (DE3)pLacI | pTrcHis2-TOPO | Food processing industry | Kamarudin et al. (2014) | |
Cold seafloor dwelling Atlantic hagfish stomach (North Norway) | Rhodococcus sp. AW25M09 | E. coli DH5-α, E. coli BL21 (DE3) | pET-22b | Food industry for preserving food flavor and integrity | De Santi et al. (2014) | |
NS | Neisseria meningitides | E. coli | pQE-30 | Production of organic esters for use in food industry | Yoo et al. (2019) | |
NS | Lactobacillus acidophilus NCFM | E. coli DH5-α, E. coli BL21 (DE3) | pET-21a | Dairy and food processing industry | Wang et al. (2018b) | |
NS | Bacillus licheniformis ATCC 14580 | E. coli BL21 | pGEMT-Easy Vector | Food processing industry | Borgi et al. (2014) | |
Pectinase | Soil (Sub-Antarctic region) | Tetracladium sp. | E. coli, P. pastoris | pPinkα-HC | Production of fruit juice and fermented beverages | Carrasco et al. (2019) |
Mannanase | Soil (China) | Bacillus subtilis Bs5 | E. coli JM109, E. coli Rosetta-gami (DE3) | pET-32a | Food and feed industry | Huang et al. (2012) |
NS not specified