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. 2021 Jul 24;38(11):4977–4986. doi: 10.1093/molbev/msab221

Fig. 2.

Fig. 2

Chromocenter disruption and micronuclei in the larval imaginal discs of lethal male D. melanogaster–D. simulans/D. mauritiana hybrids. (A) FISH against the (AATAACATAG)n satellite (green) and the (GAACAGAACATGTTC)n satellite (magenta) on larval imaginal discs from the indicated species and hybrids and costained with DAPI (blue) and Hts (gray). (B) Box-and-whisker plot of total number of (AATAACATAG)n and (GAACAGAACATGTTC)n foci per cell from D. mel y w (n = 46), D. mau (n = 64), and male (n = 43) and female (n = 60) D. mel y w×D. mau hybrids. n indicates the number of imaginal disc cells analyzed, **** represents P < 0.0001 based on Tukey’s multiple comparisons test from an ordinary one-way ANOVA and crosshairs mark the mean. (C) Box-and-whisker plot of total number of (AATAT)n foci per cell from D. mel y w (n = 48), male (n = 37) and female (n = 51) D. mel y w×D. sim C167.4 hybrids, and male (n = 39) and female (n = 41) D. mel y w×D. mau hybrids. n indicates the number of imaginal disc cells analyzed, ** represents P = 0.0049, and **** represents P < 0.0001 based on Tukey’s multiple comparisons test from an ordinary one-way ANOVA and crosshairs mark the mean. (D) IF against Lamin (green) in larval imaginal discs from the indicated species and hybrids and costained with DAPI (magenta) and phalloidin (blue). (E) Quantification of micronuclei-containing cells in the larval imaginal discs from the indicated species and hybrids. The percentage of micronuclei-containing cells is indicated above the respective columns. n indicates the number of imaginal disc cells analyzed and **** represents P < 0.0001 from Fisher’s exact test. All scale bars are 5 µm, yellow dashed lines demarcate nuclear boundary, and white dashed lines indicate cell boundary.