Table 1.
Lethal Concentrations (CL, μM) of Crem-5 and Its Mutants against Fungi.
| Aspergillus niger | Aspergillus nidulans A28 | CA 2.4138 | CA B16 | CA GD18 | CA S068 | ||
|---|---|---|---|---|---|---|---|
| Crem-5 | 4.03 | W.A. | 57.96 | 35.05 | 57.96 | 89.62 | |
| E15A | 2.74 | 7.25 (+>5.9) | 15.52 (+2.7) | 8.93 (+2.9) | 12.45 (+3.7) | 15.16 (+4.9) | |
| E15D | 5.07 | W.A. | 77.98 | 44.69 | 44.70 | 71.63 | |
| E15F | 0.87 (+3.6) | 10.98 (+>3.6) | 47.60 | 7.64 (+3.6) | 19.85 (+1.9) | 17.22 (+4.2) | |
| E15H | 0.94 (+3.3) | 8.87 (+>4.6) | 7.93 (+6.3) | 5.02 (+6.0) | 7.60 (+6.6) | 9.55 (+8.4) | |
| E15I | 2.11 | 14.08 (+>2.6) | 35.26 | 15.16 (+1.3) | 24.46 (+1.4) | 26.75 (+2.4) | |
| E15K | 1.35 (+2.0) | 4.26 (+>10.7) | 5.35 (+9.8) | 3.35 (+9.5) | 5.35 (+9.8) | 5.35 (+15.8) | |
| E15K/G36A | 7.58 | 17.91 | 51.21 | 28.11 | 44.99 | 68.23 | |
| E15M | 1.31 (+2.1) | 4.39 (+>10.4) | 8.06 (+6.2) | 3.54 (+8.9) | 7.90 (+6.3) | 8.37 (+9.7) | |
| E15N | 1.58 (+1.6) | 7.05 (+>6.1) | 12.45 (+3.7) | 6.24 (+4.6) | 10.58 (+4.5) | 8.06 (+10.1) | |
| E15P | 2.69 | W.A. | 39.60 | 16.87 (+1.1) | 28.75 (+1.0) | 35.17 (+1.5) | |
| E15Q | 2.39 | 10.85 (+>3.6) | 21.39 (+1.7) | 11.29 (+2.1) | 21.39 (+1.7) | 19.59 (+3.6) | |
| E15R | 0.70 (+4.7) | 6.24 (+>7) | 8.85 (+5.5) | 8.06 (+3.3) | 6.88 (+7.4) | 7.58 (+10.8) | |
| E15S | 3.33 | 8.59 (+>4.8) | 17.86 (+2.2) | 7.29 (+3.8) | 21.83 (+1.7) | 21.27 (+3.2) | |
| E15T | 3.52 | 7.85 (+>5.4) | 18.59 (+2.1) | 8.24 (+3.3) | 14.46 (+3.0) | 15.52 (+4.8) | |
| E15V | 2.92 | 8.84 (+>4.7) | 53.50 | 17.86 (+1.0) | 29.39 (+1.0) | 28.75 (+2.1) | |
| E15Y | 1.77 (+1.3) | 7.23 (+>5.9) | 16.78 (+2.5) | 7.93 (+3.4) | 11.49 (+4.0) | 12.65 (+6.1) | |
Note.—Fold increase is calculated as (CL of Crem-5−CL of a mutant)/CL of a mutant, in which the CL of Crem-5 on A. nidulans A28 was treated as >50 μM. Only >1-fold increase is marked by plus sign.
CA, Candida albicans; W.A., weak activity, indicating that only very small inhibition zones were observed at 2.0 nmol peptide/well.