Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 30;19:352. doi: 10.1186/s12951-021-01098-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Optimization of the concentration of AINs used for treatment of L-ADSCs. A Cell viability of L-ADSCs after treatment with AINs (n = 4, **p < 0.01 versus no treatment group). B Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay examining AIN treated L-ADSCs (blue: nucleus, green: apoptotic cell). White arrows indicate cells undergoing apoptosis that was induced by AINs. Scale bar: 100 μm. C Fluorescein diacetate/ethidium (FDA/EB) staining of L-ADSCs post-treatment with AINs (green: live cells, red: dead cells). Scale bar: 100 μm. D DiI staining of L-ADSCs treated with AINs (blue: nucleus, red: cellular membrane). Scale bar: 100 μm. E Relative mRNA expression levels of VEGF and FGF2 in L-ADSCs at 3, 6, 9, and 12 h after treatment with various concentration of AINs (n = 4, *p < 0.05 and **p < 0.01 versus no treatment group, ^#p < 0.05 and ^##p < 0.01 versus each group). Relative mRNA expression of F the apoptosis-related genes BAX and CASPASE3 and G the proliferation-related genes KI-67 and PCNA in L-ADSCs after treatment with various concentrations of AINs (n = 3, **p < 0.01 and ***p < 0.001 versus no treatment group)