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. 2021 Oct 30;12:557. doi: 10.1186/s13287-021-02623-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Leptin enhanced the expression of megalin of rBMMSCs. a Relative mRNA expression of rBMMSCs megalin in growth medium at 8 h; b IF showed leptin enhanced the protein expression of rBMMSCs megalin; c relative quantitative analysis of megalin IF staining; d 25(OH)D₃ had no effect on the relative mRNA expression of megalin with or without leptin in osteoblast differentiation medium (ODM) after 8 h; e IF showed 25(OH)D₃ did not affect the protein expression of megalin; f relative quantitative analysis of megalin IF staining. (Scale bars = 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001 in comparison with the control group.)