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. 2021 Oct 30;12:557. doi: 10.1186/s13287-021-02623-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The expression of megalin promoted by leptin through PI3K/AKT-dependent CMA was partially blocked by PI3K/AKT signal pathway inhibitor LY294002 (20 μM). a Western blot analyses showed that the level of p-AKT was significantly decreased in leptin-treated rBMMSCs with the inhibitor in ODM at 1 h; b relative quantitative analysis of western blot analyses; c the mRNA relative expression of LAMP2A and HSC70 inhibited by leptin combined with 25(OH)D₃ was partially rescued in ODM at 8 h; d the decreased protein levels of LAMP2A and HSC70 induced by leptin and 25(OH)D₃, which were reversed by the inhibitor LY294002 (20 μM) at 24 h; e relative quantitative analysis of western blot analyses; f the mRNA relative expression of megalin by leptin with 25(OH)D₃ + LY294002 was significantly decreased; g IF showed that the expression level of megalin enhanced by leptin and 25(OH)D₃, which was significantly inhibited by the inhibitor LY294002 (20 μM) at 24 h; h relative quantitative analysis of megalin IF staining. (Scale bar = 100 μm, *P < 0.05, **P < 0.01, ***P < 0.001 in comparison with the control group; #P < 0.05, ##P < 0.01 in comparison with leptin + 25(OH)D₃ group.)