Table 1.
Sequences of primers
| Gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| Megalin | ACAACTCGGATGAACGGGAC | AGTAGGTGCCGTTGGGAAAG |
| ALP | AGATGGATGAGGCCATCGGA | CCAAACGTGAAAACGTGGGA |
| COL1A1 | CACTGCAAGAACAGCGTAGC | AAGTTCCGGTGTGACTCGTG |
| RUNX2 | CAGACCAGCAGCACTCCATA | AGACTCATCCATTCTGCCGC |
| OSX | GGTCCTGGCAACACTCCTAC | AAGAGGTGGGGTGCTGGATA |
| OSP | GAGACCATGCAGAGAGCGAG | TTGACCTCAGTCCGTAAGCC |
| LAMP2A | AAGAGCAGGTGGTTTCCGTG | ATGGGCACAAGGAAGTTGTCT |
| HSC70 | CTCCATTACCCGTGCTCGAT | GAACCACCCACCAGGACAAT |
| LepR | TTGGTCCTCTTCTGGAGC | CCCCTTGTGGAATCTGGAGTG |
| β-actin | CTCTGTGTGGATTGGTGGCT | CGCAGCTCAGTAACAGTCCG |
ALP alkaline phosphatase, COL1A1 collagen type I, RUNX2 runt-related transcription factor 2, OSX osterix, OSP osteopontin, LAMP2A lysosomal-associated membrane protein 2A, HSC70 heat-shock cognate 71 kDa protein, LepR leptin receptor