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. 2021 Oct 30;14:50. doi: 10.1186/s13072-021-00424-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Information obtained from the differential centrifugation assay. In this assay, chromatin is combined with MgCl₂ to the indicated value, pelleted in a microfuge for 10 min, and A₂₆₀ of the supernatant measured. Data are expressed as the fraction of the chromatin in the supernatant as a function of MgCl₂ concentration. Results are shown for native 12-mer nucleosomal arrays (red points and line). Factors that shift the native curve to the left or right are indicated