Table 2.

Sequence alignment of the BRCA1 and BRCA2 proteins for the two missense VUS identified

SPECIES	MATCH	GENE	AA	ALIGNMENT
BRCA1 c.5348T>C (p.Met1783Thr)
Human			1783	T	N	M	P	T	D	Q	L	E	W	M	V	Q	L	C	G	A	S	V	V	K	E	L
mutated	Not conserved		1783	T	N	M	P	T	D	Q	L	E	W	T	V	Q	L	C	G	A	S	V	V	K	E
P. troglodytes	All identical	ENSPTRG00000009236	1783	T	N	M	P	T	D	Q	L	E	W	M	V	Q	L	C	G	A	S	V	V	K	E
M. mulatta	All identical	ENSMMUG00000001329	1783	T	N	M	P	T	D	Q	L	E	W	M	V	Q	L	C	G	A	S	V	V	K	E
F. catus	No homologue
M. musculus	All identical	ENSMUSG00000017146	1726	T	N	M	P	K	D	E	L	E	R	M	L	Q	L	C	G	A	S	V	V	K	E
G. gallus	All conserved	ENSGALG00000002781	1674	T	D	M	T	T	G	H	L	E	W	I	V	E	L	C	G	A	S	V	V	K	Q
T. rubripes	All identical	ENSTRUG00000009091	1207	T	D	M	T	T	A	E	M	E	L	M	V	E	L	C	G	A	T	V	V	K	D
D. rerio	no homologue
D. melanogaster	no homologue
C. elegans	No homologue
X. tropicalis	All identical	ENSXETG00000024564	1524	T	D	M	T	L	D	D	L	E	W	M	V	S	E	C	G	S	T	V	V	R	D
BRCA2 c.7504C>T (p.Arg2502Cys)
Human			2502	D	M	R	I	K	K	K	Q	R	Q	R	V	F	P	Q	P	G	S	L	Y	L	A
mutated	Not conserved		2502	D	M	R	I	K	K	K	Q	R	Q	C	V	F	P	Q	P	G	S	L	Y	L	A
P. troglodytes	All identical	ENSPTRG00000005766	2502	D	M	R	I	K	K	K	Q	R	Q	R	V	F	P	Q	P	G	S	L	Y	L	A
M. mulatta	no homologue
F. catus	No homologue
M. musculus	Not conserved	ENSMUSG00000041147	2423	D	M	R	I	K	N	K	E	R	R	H	L	R	L	Q	P	G	S	L	Y	L	T
G. gallus	Not conserved	ENSGALG00000017073	2457	E	M	R	I	K	K	K	Y	R	Q	N	I	S	P
T. rubripes	No homologue
D. rerio	not conserved	ENSDARG00000079015	2046	D	M	R	L	R	K	K	K	R	Q	T	I	R	P	V	P	G	S	L	Y	L	A
D. melanogaster	No homologue
C. elegans	No homologue
X. tropicalis	All conserved	ENSXETG00000017011	2293	E	M	R	I	R	K	K	L	R	Q	K	I	K	P	H	P	G	S	L	Y	R	L