Table 1.

Primer sequences used for mRNA quantification by RT-qPCR

	Forward primer 5’–3’	Reverse primer 5’–3’
UCP1	ACTGTACAGAGCTGGTGACA	TGGTAGAGAGTTGATGAATCTCGT
PGC1α	GTGGATGAAGACGGATTGCC	TTCTGAGTGCTAAGACCGCT
PRDM16	GGACAGTGACAGAGACAAAAGC	CTGTGAATAGAAGGCCGGTA
TMEM26	TTGCCATGGGCTAGAATCCG	TAAAGGCCTGTGCAGCTACC
PPARγ	ATCAGGTTTGGGCGAATG	TTTGGTCAGCGGGAAGGA
β3-AR	TGCTGTTCCTTTGCCTCCAA	TAGCTACGACGAACACTCGA
GAPDH	GGCTCTCTGCTCCTCCCTGTTCTA	CGTCCGATACGGCCAAATCCGT

UCP1 uncoupling protein 1, PGC1α peroxisome proliferator-activated receptor γ coactivator-1α, PRDM16 positive regulatory domain containing 16, TMEM26 transmembrane protein 26, PPARγ peroxisome proliferator-activated receptor γ, β3-AR β3-adrenergic receptor, GAPDH glyceraldehyde-3-phosphate dehydrogenase