Table 8.

Effect of bismuth subsalicylate and calcium ammonium nitrate on final liver mineral concentration, liver mineral concentration change, and rectal temperature.

Item	Treatment1					SEM2	P-value3
	U	NIT	UBS	NITBS	LOW-S		SUL	NPNS	BSS	BSS × NPNS
Final liver mineral4, mg/kg DM
Se	1.81	1.27	1.28	1.27	1.68	0.193	0.619	0.149	0.156	0.160
Fe	397.91	298.65	400.27	372.50	407.76	54.277	0.211	0.211	0.455	0.479
Cu	134.35	89.95	61.34	33.26	171.98	30.566	0.384	0.251	0.042	0.794
Zn	133.99	114.17	103.89	105.18	112.87	12.878	0.218	0.454	0.145	0.403
Mn	10.71	7.93	7.96	8.11	9.25	0.923	0.233	0.142	0.153	0.103
Liver mineral change5, mg/kg DM
Se	−0.31	−0.93	−0.81	−1.06	−0.62	0.225	0.322	0.059	0.161	0.421
Fe	62.84	30.64	33.67	−16.00	184.02	67.689	0.207	0.717	0.750	0.916
Cu	−131.81	−180.81	−186.72	−266.32	−125.55	38.894	0.906	0.100	0.073	0.690
Zn	2.00	−13.66	−32.67	−39.41	−22.31	13.010	0.181	0.391	0.025	0.732
Mn	1.42	−1.32	−1.36	−1.89	−0.24	1.123	0.293	0.156	0.148	0.333
Rectal temperature, °C	38.93	39.09	38.91	39.00	39.04	0.081	0.356	0.132	0.536	0.736

¹U, Control (Urea at 0.68% with 0.28% RAS); NIT, Nitrate (eCAN at 2.0% with 0.28% RAS); UBS, Urea-Bismuth (Urea at 0.68% and BSS at 0.33% with 0.28% RAS); NITBS, Nitrate-Bismuth (eCAN at 2.0% and BSS at 0.33% with 0.28% RAS); LOW-S, Low sulfur (Urea at 0.70% with 0.14% RAS).

²The highest SEM was included in this table (n = 10 experimental units/treatment mean).

³Significance is declared at P < 0.05, tendencies are discussed at 0.05 > P < 0.10. SUL = Effect of sulfur; NPNS = Effect of non-protein nitrogen source; BSS = Effect of BSS; BSS × NPNS = Interaction between NPNS and BSS.

⁴Final liver mineral was analyzed using initial liver mineral concentration as a covariate.

⁵Change in liver mineral status was calculated by subtracting final mineral concentration minus initial mineral concentration.