TABLE 1.
sRNA candidates in B. pertussis
| Namea | Rankb | ANNOgesic IDb | Neighboring genesc |
Directiond | Expression modee |
|||
|---|---|---|---|---|---|---|---|---|
| ANNOgesicb | Northern blotf | |||||||
| 5’ ORF | 3’ ORF | |||||||
| S1 | 4 | 212 | <BP2496 | BP2497> | < | Bvg(−) | Bvg(−)/Bvg(+) | |
| S2 | 5 | 128 | BP1548> | dnaX> | > | Bvg(−) | Independent | |
| S3 | 6 | 188 | <ndk | valS> | < | Independent | Independent | |
| S4 | 7 | 296 | BP3391> | <BP3392 | > | Independent | Independent | |
| S5 | 8 | 16 | <phoB | ubiE> | < | Bvg(+) | ||
| S6 | 104 | 294 | <BP3385 | <BP3386 | < | Bvg(−) | ||
| S7 | 11 | 168 | <BP1907 | cysS > | > | Independent | ||
| S8 | 16 | 123 | <BP1496 | thrS> | > | Independent | Bvg(+) | |
| S9 | 17 | 45 | BP0470> | ribB> | > | Bvg(+) | Bvg(+) | |
| S10 | 25 | 298 | <BP3409 | <BP3410 | < | Bvg(+) | Bvg(+) | |
| S11 | 26 | 242 | <BP2886 | BP2887> | > | Bvg(+) | Bvg(+) | |
| S12 | 28 | 17 | ubiE> | BP0162> | > | Independent | Bvg(−)/Bvg(+) | |
| S13 | 30 | 239 | panB> | BP2851> | < | Bvg(−) | ||
| S14 | 34 | 95 | BP1164> | BP1165> | > | Independent | ||
| S15 | 86 | 288 | BP3352> | BP3353> | < | Independent | ||
| S16 | 52 | 42 | <BP0405 | <tRNA Val | < | Bvg(+) | ||
| S17 | 9 | 276 | <BP3151 | <BP3152 | < | Bvg(+) | Bvg(+) | |
| S18 | <BP2735 | BP2736> | < | Bvg(−) | ||||
| S19 | <dnaA | rpmH> | > | |||||
| S20 | <BP0920 | <citB | > | Independent | ||||
Names in bold were confirmed by Northern blot analyses.
ANNOgesic rank, ID, and expression mode (see Table S1). S18, S19, and S20 were not identified by ANNOgesic so this information is not available for them.
Neighboring genes of the sRNA; > corresponds to top strand gene; < corresponds to bottom strand gene.
Directionality of the sRNA transcription; >, top strand; <, bottom strand.
Expression mode of sRNA in RNA-seq or Northern blot analyses. Bvg(+), observed in the presence of BvgA∼P; Bvg(−), observed in the absence of BvgA∼P; (−), not detected; CONST, similar levels in BvgA(+) and BvgA(−).
Northerns were performed for sRNAs in bold.