TABLE 2.

List of the most frequent mutations in all samples and those unique for each geographic location^a

Nucleotide mutation	Amino acid mutation	Geo	No. of:		Gene	Protein	Synonymity	Amino acid alteration property
			Samples with mutation	GISAID events
Most frequentb
C241T	Extragenic	All	65	7	5′ UTR	NA	NA	NA
A23403G	D614G	All	65	6	S gene	Spike	Non-S	Radical
C14408T	P4715L	All	62	8	ORF1ab	NSP12/RNApol	Non-S	Radical
C3037T	F924F	All	59	10	ORF1ab	NSP3	S	NA
G25563T	Q57H	All	59	9	ORF3a	ORF3a	Non-S	Conservative
C1059T	T265I	All	56	13	ORF1ab	NSP2	Non-S	Conservative
G29540A	Extragenic	All	16	2	N/ORF10 linking region	NA	NA	NA
C11916T	S3884L	All	15	4	ORF1ab	NSP7/replicase	Non-S	Radical
C18998T	A6245V	All	15	1	ORF1ab	NSP14/NSP11	Non-S	Conservative
Uniquec
G29540A	Extragenic	NYC	16	2	N/ORF10 linking region	NA	NA	NA
C11916T	S3884L	NYC	15	4	ORF1ab	NSP7/replicase	Non-S	Radical
C18998T	A6245V	NYC	15	1	ORF1ab	NSP14/NSP11	Non-S	Conservative
C4113T	A1283V	NYC	4	6	ORF1ab	NSP3	Non-S	Conservative
C27964T	S24L	TN	7	7	ORF8	ORF8	Non-S	Radical
C3411T	A1049V	TN	7	3	ORF1ab	NSP3	Non-S	Conservative
T6394C	D2043D	TN	7	3	ORF1ab	NSP3	S	NA
C10319T	L3352F	TN	7	4	ORF1ab	NSP5	Non-S	Conservative
A4197G	E1311G	TN	4	2	ORF1ab	NSP3	Non-S	Radical
G8179A	R2638R	TN	4	4	ORF1ab	NSP3	S	NA
C15924T	Y5220Y	TN	4	1	ORF1ab	NSP12/RNApol	S	NA

^aGeo, geographic location; UTR, untranslated region; NA, not applicable; Non-S, nonsynonymous; S, synonymous; NSP, nonstructural protein; RNApol, RNA polymerase; NYC, New York City; TN, Tennessee.

^bMost frequent mutations in all samples.

^cUnique mutations for each geographic location.