FIG 5.

Polymyxin B tolerance increases after exposure to CP. (A) Heat map of membrane-remodeling proteins under all metal depletion conditions. Protein expression is compared to the metal-replete condition. The log₂ fold change (LFC) of each significantly (P < 0.05) changed protein is shown. (B) The minimal bactericidal concentration (MBC) of polymyxin B was determined for PA14 after preculture in metal-replete, metal-depleted, and low-Mg(II) CDM and after CP treatment. The geometric mean of the three biological replicates for each condition is denoted with a line. The biological replicates are represented with circles (replicate 1), squares (replicate 2), and triangles (replicate 3). Significance was determined by one-way ANOVA with Dunnett’s posttest; *, P < 0.05.