Gut microbiota-based prediction of the clinical weight loss parameters after LCD treatment by ANN. A high-accuracy ANN prediction model was established using the relative abundance of gut microbiota at the genus level and weight loss parameters, including the baseline value of BMI, waist, WHR, BFR, and VFA after LCD treatment (A to E), changes in weight loss parameters (F to J), and the ratio of changes in these parameters to their baseline value in 5-fold cross-validation (K to O).