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. 2021 Sep 29;9(2):e00687-21. doi: 10.1128/Spectrum.00687-21

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Copyright © 2021 Sun et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Identification of the epitope containing a short peptide specific for H7 subtype AIV by indirect immunofluorescence assay. CEF cells were infected with different subtypes of AIVs and fixed with methanol. After incubation with chicken antiserum against H7-12 peptide and FITC-conjugated goat anti-chicken IgY antibody, fluorescence was observed under a fluorescence microscope. (A to J) H7 AIV JD/17 with H7 AIV antiserum as positive control (A), H7 AIV JD/17 (B), H7 AIV JD/17 with unvaccinated SPF chicken serum as negative control (C), H1 AIV (D), H3 AIV (E), H4 AIV (F), H5 AIV (A/Chicken/Huadong/ZJ0104/16) (G), H6 AIV (H), H9 AIV (A/Chicken/Shanghai/F/98) (I), and H10 AIV (J). AIV strains are described in Table 1.