FIG. 6.

(A) hGAR1 cDNA and predicted protein sequences. Nucleotide positions are given on the left, and amino acids are numbered on the right. The open reading frame (nt 265 to 915) encodes a protein of 217 amino acids. Sequences of the GAR domains are in bold. Two in-frame termination codons of the 5′ untranslated region are underlined. A putative polyadenylation signal is double underlined. (B) Increasing dilutions of affinity-purified antipeptide Abs αP795 and αP796 were used for Western blotting of HeLa cell extracts in a multiscreen apparatus. Sizes are indicated in kilodaltons.