TABLE 5.
Most important cross-species putative gene targets in B. cinerea and F. graminearum, their expression pattern and putative function
| milRNA identifier | Gene target transcript ID | Locus ID (gene name) | Expression (log2FC) | Target gene family | Characterized or putative function |
|---|---|---|---|---|---|
| Botrytis cinerea | |||||
| cro-mir-1, cro-mir-2, and cro-mir-6 | XM_024690817 | Bcin_01g09230 (bcphy2) | 3.53 | Protein kinase | Signal transduction |
| cro-mir-9 | XM_024690817 | Bcin_05g05430 | 3.38 | Phospholipid methyltransferase | Lipid metabolic process (membrane lipid biogenesis) |
| cro-mir-13 | XM_001553702 | Bcin_02g04090 | 2.9 | Fungal 1,3(4)-β-d-glucanases | Glucan catabolic process |
| cro-mir-13 and cro-mir-2 | XM_001547426 | Bcin_01g00360 (bcerg1) | 2.74 | Squalene monooxygenase | Sterol biosynthetic process |
| cro-mir-4 | XM_001557947 | Bcin_12g00180 (bccap9) | 2.69 | Aspartic proteases of fungal origin | Proteolysis, induced during infection |
| cro-mir-5 | XM_001557734 | Bcin_04g06150 | 2.29 | Cyclase (Lanc-like super family) | Biosynthesis of lantibiotics |
| cro-mir-1 and cro-mir-2 | XM_024693876 | Bcin_07g01580 (bcnog1) | 2.27 | GTP-binding protein | Ribosomal large subunit biogenesis |
| cro-mir-4 | XM_024693364 | Bcin_06g01930 (bcgo1) | 1.87 | Chitin binding | Chitin recognition |
| cro-mir-5 | XM_001561274 | Bcin_01g06010 (bccrh1) | 1.83 | Glycosylphosphatidylinositol-glucanosyltransferase | Fungal cell wall biosynthesis |
| cro-mir-5 | XM_024691832 | Bcin_03g02630 (bcste11) | 1.81 | Protein kinase | Signal transduction, virulence |
| cro-mir-36 | XM_024691483 | Bcin_02g06930 | 1.67 | 1,3-β-d-Glucan synthase | Glucan biosynthesis |
| cro-mir-36 | XM_001558808 | BCIN_02g02410 | 1.61 | Glycosyl hydrolase | Fungal-type cell wall polysaccharide metabolic process |
| cor-mir-11 | XM_001551241 | BCIN_14g02820 | 1.57 | β-Glucan synthesis-associated protein | Fungal cell wall biosynthesis |
| cro-mir-11 | XM_001550300 | BCIN_05g00350 (bcnoxA) | 1.57 | NADPH oxidase (NOX) | Pathogenicity, fusion of conidial anastomosis tubes, and formation of sclerotia and conidia |
| cro-mir-4 | XM_024690414 | BCIN_01g03790 (bcchsIV) | 1.54 | Chitin synthase | Cell wall biosynthesis, development and pathogenicity |
| cro-mir-4 | XM_024692792 | BCIN_05g00540 (bchhk1) | 1.47 | Protein kinase | Signal transduction b |
| cro-mir-13 and cro-mir-2 | XM_001551683 | BCIN_09g06130 (bcpls1) | 1.4 | Tetraspanins | Appressorium development, host penetration |
| cro-mir-1 and cro-mir-2 | XM_001547152 | BCIN_12g05700 | 1.38 | Cyclases | Biosynthesis of lantibiotics |
| cro-mir-36 | XM_001554608 | BCIN_08g03180 | 1.26 | Transcription factor | Appressorium formation |
| cro-mir-36 | XM_024694081 | BCIN_07g04590 (bchts1) | 1.2 | Histidine-tRNA ligase | Translation, ribosomal structure, and biogenesis |
| cro-mir-4 and cro-mir-36 | XM_024695521 | BCIN_10g02810 (bcyta7) | 1.13 | Bromodomain-containing protein | Chromatin remodeling |
| ro-mir-1 and cro-mir-2 | XM_024694912 | BCIN_09g01210 (bcchs1) | 1.11 | Chitin synthase | Cell wall biosynthesis, virulence |
| cro-mir-13 | XM_024697868 | BCIN_16g01130 (bcpka1) | 1.03 | Serine/threonine kinases | Conidial germination, growth, and virulence |
| cro-mir-5 | XM_024694566 | BCIN_08g03270 (bcvac8) | 1.02 | Fungus-type vacuole membrane | Intracellular trafficking and secretion |
| Fusarium graminearum | |||||
| cro-mir-3 | XM_011328464 | FGSG_07067 | 1.41 | Transcription factor | Virulence |
| cro-mir-4 | XM_011319656 | FGSG_02083 | 1.02 | Transcription factor | Mycotoxin biosynthesis |
| cro-mir-5 | XM_011317736 | FGSG_00376 | 1.07 | Ubiquinone oxidoreductase | Virulence |
| cro-mir-5 | XM_011321023 | FGSG_13747 | 1.03 | Membrane transporter | Transmembrane transporter activity |
| cro-mir-5 | XM_011329154 | FGSG_07665 | 1.14 | Membrane transporter | Transmembrane transporter activity |
| cro-mir-1 and cro-mir-2 | XM_011319110 | FGSG_11973 | 1.44 | Membrane transporter | Transmembrane transporter activity |
| cro-mir-9 | XM_011329717 | FGSG_09686 | 1.58 | Vesicle-mediated transport | Intracellular trafficking and secretion |
| cro-mir-6 | XM_011326744 | FGSG_06384 | 1.11 | Vesicle-mediated transport | Intracellular trafficking and secretion |