TABLE 6.
Differential expression patterns of selected genes in B. cinerea and F. graminearum during interaction with Δdcl1 and Δdcl2 mutants compared to those of wild-type C. rosea and the same mycohost
| GenBank accession no. | Locus tag (gene ID) | Gene function | Expression (log2FC)a |
Biological function | |
|---|---|---|---|---|---|
| Δdcl1 | Δdcl2 | ||||
| Botrytis cinerea | |||||
| XM_001547559 | BCIN_02g08360 (bcfrq1) | Circadian oscillator | 1.05 | 2.03 | Virulence |
| XM_001550300 | BCIN_05g00350 (bcnoxa) | NADPH oxidase complex | −0.39 | 1.57 | Virulence |
| XM_001552181 | BCIN_12g03770 (bcnop53) | Pre-rRNA processing factor | 0.19 | 1.59 | Fungal development and pathogenesis |
| XM_001555445 | BCIN_03g06840 (bcnoxr) | Regulatory subunit of NOX (NADPH oxidase regulator) | −0.01 | 1.56 | Differentiation and pathogenicity |
| XM_024691832 | BCIN_03g02630 (bcste11) | MAPK triple kinase | 0.16 | 1.81 | Hyphal growth |
| XM_024693262 | BCIN_06g00026 (mfsG) | Major facilitator superfamily transporter | −0.84 | −5.95 | Tolerance to glucosinolate-breakdown products, required for pathogenicity |
| XM_024697209 | BCIN_14g03930 (bcltf1) | GATA transcription factor | 1.66 | 3.86 | Tolerance to oxidative stress, virulence |
| XM_024697551 | BCIN_15g03390 (bcvel1) | Regulatory protein of the VELVET complex | 0.13 | 1.59 | Formation of oxalic acid, virulence |
| XM_024694938 | BCIN_09g01620 (bccry2) | DNA photolyase | 1.74 | 3.57 | Negative regulation of filamentous growth and conidiation |
| XM_001561274 | BCIN_01g06010 (bccrh) | Transglycosylase | 0.00 | 1.83 | Cell wall biogenesis, virulence |
| XM_024693846 | BCIN_07g01300 (bcchsvii | Chitin synthase | 0.06 | 1.83 | Cell wall biogenesis, virulence |
| XM_024696504 | BCIN_12g05360 (bcchsvi) | Chitin synthase | 0.04 | 1.66 | Cell wall biogenesis, Virulence |
| XM_001545464 | BCIN_12g05370 (bcchsv) | Chitin synthase | −0.12 | 1.63 | Cell wall biosynthesis |
| XM_024690414 | BCIN_01g03790 (bcchsiv) | Chitin synthase | −0.15 | 1.54 | Cell wall biosynthesis |
| XM_001554790 | BCIN_03g09000 | Septin GTPase | 2.87 | 5.60 | Cytoskeleton-dependent cytokinesis (septin ring) |
| XM_024693922 | BCIN_07g02420 | MFS transporters | −0.83 | 2.99 | Xenobiotic transport |
| XM_024695797 | BCIN_11g00800 | Protein kinase CK2 | 1.43 | 2.96 | Regulates various cellular processes |
| XM_024690261 | BCIN_01g01760 | Chitinase activity | 0.06 | 2.67 | Cell wall biosynthesis |
| XM_024696411 | BCIN_12g03920 | Chitin binding | 0.35 | 2.22 | Chitin recognition |
| XM_001549884 | BCIN_01g02970 | Chitin binding | 0.00 | 1.96 | Chitin recognition |
| XM_024693364 | BCIN_06g01930 (bcgo1) | Chitin binding | −0.07 | 1.87 | Chitin recognition |
| XM_001547426 | BCIN_01g00360 (bcerg1) | Squalene monooxygenase | 1.55 | 2.74 | Sterol biosynthetic process |
| Fusarium graminearum | |||||
| XM_011317671 | FGSG_00324 (fgmyt3) | Transcription factor | +1.05 | +1.52 | Fungal development and pathogenicity |
| XM_011318135 | FGSG_00729 (gzhmg005) | Transcription factor | +0.99 | +1.56 | Virulence |
| XM_011320684 | FGSG_10057 (fgerb1) | Transcription factor | +1.44 | +1.52 | Growth and pathogenicity |
| XM_011321826 | FGSG_08617 (gzc2h066) | Transcription factor | +1.46 | +1.84 | Virulence |
| XM_011322702 | FGSG_04580 (fgabc1) | ABC pleiotropic drug resistance transporter | +1.72 | 0.40 | Virulence and tolerance to benalaxyl |
| XM_011327033 | FGSG_11028 | Multidrug resistance-associated protein | +2.65 | Nivalenol biosynthesis | |
| XM_011326203 | FGSG_05898 (fgplc1) | Fungal phospholipase C | +1.31 | +1.66 | Development, pathogenicity, and stress responses |
| XM_011328541 | FGSG_07133 (gzzc230) | Transcription factor | +1.18 | +1.72 | Virulence |
| XM_011329465 | FGSG_07928 (gzc2h059) | Transcription factor | +1.29 | +1.61 | Virulence |
| XM_011317284 | FGSG_00007 | Cytochrome P450 | −3.85 | −3.68 | DON biosynthesis |
| XM_011317365 | FGSG_00071 (tri1) | Cytochrome P450 | −1.62 | −1.38 | DON biosynthesis |
| XM_011323873 | FGSG_03534 (tri3) | 15-O-Acetyltransferase | −2.99 | −4.17 | DON biosynthesis |
| XM_011323872 | FGSG_03535 (tri4) | Trichodiene oxygenase | −3.24 | −5.12 | DON biosynthesis |
| XM_011323870 | FGSG_03537 (tri5) | Trichodiene synthase | −2.74 | −3.56 | DON biosynthesis |
| XM_011323871 | FGSG_03536 (tri6) | Transcription factor | −1.15 | −1.65 | DON biosynthesis |
| XM_011323868 | FGSG_03539 (tri9) | TRI9 protein | −1.42 | −1.84 | DON biosynthesis |
| XM_011323864 | FGSG_03543 (tri14) | Mala s 1-allergenic | −2.67 | −3.91 | DON biosynthesis |
| XM_011323865 | FGSG_03542 | Cytochrome P450 | −1.81 | −5.13 | DON biosynthesis |
| XM_011322312 | FGSG_08196 | Peptidase A4 | −3.30 | −5.00 | Highly induced in mycotoxin-inducing media |
| XM_011324413 | FGSG_03065 (gzcarb) | Phytoene dehydrogenase | −0.80 | −2.08 | Neurosporaxanthin and torulene BGC |
| XM_011324406 | FGSG_03071 | FAD-dependent oxidoreductase | −1.74 | −3.26 | Neurosporaxanthin and torulene BGC |
| XM_011324412 | FGSG_03066 (gzcara) | al-2/carRA phytoene synthase | −0.77 | −1.58 | Neurosporaxanthin and torulene BGC |
| XM_011321137 | FGSG_10460 (fsl5) | Enoyl reductase | 1.10 | −4.27 | Fusarielin BGC |
| XM_011321139 | FGSG_10462 (fls3) | Aldose 1‐epimerase | 1.54 | −2.45 | Fusarielin BGC |
| XM_011321140 | FGSG_10463 (fls2) | Esterase | 1.78 | −2.03 | Fusarielin BGC |
| XM_011321141 | FGSG_10464 (fls1) | Polyketide synthase | 1.52 | −1.87 | Fusarielin BGC |
a
Significant differences (FDR < 0.05 and log2FC > 1.5 or <−1.5) are highlighted in boldface letters.