FIG 1.
Obtaining adaptors from plates. (A and B) Susceptibility of parent strain H99 to fluconazole (FLC) was measured by Etest (A) and spot assay (B). For Etests, amounts of 1 × 105 cells were spread on YPD plates. An Etest strip was applied to the center of the plate. In spot assays, cells were suspended in distilled water and serially diluted by 10-fold. Three microliters of each dilution was spotted on the plates containing the different drug concentrations as indicated in the figure. Plates were incubated at 30°C for 3 days and then photographed. (C) Approximately 1 × 106 cells were spread on YPD plates supplemented with FLC concentrations as indicated in the figure. Plates were incubated at 30°C and photographed after 5 days (control and 32-μg/ml FLC plate) or 15 days (64-μg/ml and 128-μg/ml plates).