FIG 5.

Cross-tolerance of aneuploids to antifungals. Fluconazole (FLC) adaptors obtained from YPD plates + FLC (≥32 μg/ml) (A) or YPD broth + FLC (8 μg/ml) (B and C) were tested for growth in FLC, amphotericin B (AMB), flucytosine (5FC), and hydrogen peroxide (H₂O₂). Adaptors representing unique karyotypes were tested. Cell were adjusted to 1 × 10⁷ cells/ml. Serial 10-fold dilutions of cell suspension was spotted (3 μl/spot) on the plates. The plates were incubated at 30°C for 3 days and then photographed. Tests of 5FC and the combination of 5FC and FLC were determined on SD plates; other drugs were tested on YPD plates.