TABLE 4.
Features of the 17 carbapenem-resistant P. aeruginosa isolates
| Isolate |
Animal species |
Clinical sample | MLST sequence type | T3SS virulotype |
Carbapenemase detection |
MIC (mg/liter)a of: |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. | Name | exoU+/exoS− | exoU−/exoS+ | CIMTris | PCR detection of blaIMP, blaNDM, blaVIM, and blaGES | IPM | IPM + PAβN | IPM + APB | IPM + PaβN + APB | MEPM | MEPM + PAβN | MEPM + APB | MEPM + PaβN + APB | |||
| 1 | CA10562 | Dog | Ear discharge | ST3014 (CC3014) | − | + | − | − | 8 | 1 | 0.5 | ≤0.25 | ||||
| 2 | CA12133 | Dog | Ear discharge | ST1600 | − | + | − | − | 64 | 4 | 8 | 1 | ||||
| 3 | CA12482 | Cat | Skin | ST198 (CC198) | − | + | − | − | 16 | 16 | 1 | 1 | ||||
| 4 | CA13227 | Dog | Ear discharge | ST209 (CC274) | − | + | − | − | 8 | 8 | 1 | 1 | ||||
| 5 | CA13620 | Dog | Ear discharge | ST277 (CC277) | − | + | − | − | 4 | 2 | 0.5 | ≤0.25 | 4 | 4 | 4 | 2 |
| 6 | CA13876 | Cat | Nasal discharge | ST1097 | − | + | − | − | 8 | 4 | 0.5 | ≤0.25 | ||||
| 7 | CA14241 | Dog | Ear discharge | ST3045 | − | + | − | − | 16 | 16 | 1 | 2 | ||||
| 8 | CA16138 | Dog | Ear discharge | ST3574b (CC262) | − | + | − | − | 8 | 2 c | 4 | 1 c | ||||
| 9 | CA16209 | Dog | Ear discharge | ST606 | + | − | − | − | 8 | 1 | 0.5 | ≤0.25 | ||||
| 10 | CA17343 | Dog | Uterine pus | ST348 (CC348) | − | + | − | − | 128 | 8 c | 4 | 0.5 c | 16 | 2 c | 8 | 2 c |
| 11 | CA17462 | Dog | Urine | ST270 | − | + | − | − | 4 | 1 | 0.5 | ≤0.25 | ||||
| 12 | CA17502 | Dog | Ear discharge | ST155 (CC155) | − | + | − | − | 16 | 4 | 8 | 4 | ||||
| 13 | CA19603 | Cat | Ear discharge | ST235 (CC235) | + | − | − | − | 32 | 16 | 2 | NGd | 8 | 1 | 4 | NGd |
| 14 | CA19802 | Dog | Urine | ST399 (CC399) | − | + | − | − | 32 | 16 | 2 | 2 | 16 | 8 | 8 | 4 |
| 15 | CA19818 | Dog | Urine | ST266 | − | + | − | − | 4 | 2 | ≤0.25 | ≤0.25 | ||||
| 16 | CA20091 | Dog | Ear discharge | ST348 (CC348) | − | + | − | − | 4 | 4 | 0.5 | 0.5 | ||||
| 17 | CA20115 | Dog | Urine | ST3135 | − | + | − | − | 4 | 8 | 0.5 | 0.5 | ||||
a
A decrease of more than 4-fold in MIC values are indicated in bold.
b
Sequence type newly assigned in this study.
c
PAβN concentration of 20 mg/liter was used.
d
No visible growth was observed in the presence of imipenem or meropenem plus 20- or 40-mg/liter PAβN and 300-mg/liter APB.