TABLE 5.
Genetic characteristics of P. aeruginosa isolates no. 2, no. 10, and no. 13
| Characteristic | Results for: |
||
|---|---|---|---|
| Isolate 2 (CA12133) | Isolate 10 (CA17343) | Isolate 13 (CA19603) | |
| Sequence type/serotype | ST1600/O3 | ST348/O2 | ST235/O11 |
| Antimicrobial resistance genes | blaPDC-3, blaOXA-937, aph(3′)-IIb, fosA, catB7 | blaOXA-494, blaPDC-5, aph(3′)-IIb, fosA, catB7, crpP | blaOXA-488, blaPDC-35, aph(3′)-IIb, fosA, catB7, qnrVC1-aac(6′)-Ib-blaOXA-10-aadA1-dfrA14, aadB-cmlA10-aadA2-sul1 |
| Amino acid substitutionsa | |||
| AmpC regulators | |||
| AmpR | Arg244Trp, Gly273Glu | Gly283Glu, Met288Arg | |
| AmpD | Ser5Phe, Gly148Ala, Asp183Tyr | Gly148Ala | |
| AmpDh2 | |||
| AmpDh3 | Ile67Thr | Ala219Thr | Ala208Val |
| MexAB-OprM regulators | |||
| MexR | Val126Glu | ||
| NalC | Gly71Glu, Ser209Arg | Glu153Gln | |
| NalD | |||
| MexCD-OprJ regulator | |||
| NfxB | Arg21His, Asp56Gly, Gly133Ser | ||
| MexEF-OprN regulators | |||
| MexS | Asp249Asn | Gly78Ser, Asp249Asn | frameshift (Δnt 362T) |
| MexT | |||
| MexXY-OprA regulator | |||
| MexZ | Frameshift (Δnt 399G) | ||
| OprD mutationsa | Thr103Ser, Lys115Thr, Phe170Leu | Premature stop codon (W65stop) | Δnt 1–75 |
| QRDR mutationa | Ser466Phe (GyrB) | ||
| Heavy metal resistance genes | copRS, copAB, czcABCD, colRS, chrA, sodA | copRS, copAB, copD, czcABCD, colRS, chrA, sodA | copRS, copAB, czcABCD, colRS, chrA, sodA |
| Virulence-associated genes | T1SS (aprA, aprX, hasAp), T2SS (lasA, lasB, plcH, plcN, plcB, prpL, phoA, lipA, lipC, loxA, toxA, paAP), T3SS (exoT, exoS, exoY), H2-T6SS (tssB2, pldB), H3-T6SS (tssB3), QS (lasI, lasR, rhlI, rhlR), others (fliC, rhlAB) | T1SS (aprA, aprX, hasAp), T2SS (lasA, lasB, plcH, plcN, plcB, prpL, phoA, lipA, lipC, loxA, toxA, cbpD, paAP), T3SS (exoT, exoS, exoY), H2-T6SS (tssB2, pldB), H3-T6SS (tssB3), QS (lasI, lasR, rhlI, rhlR), others (fliC, rhlAB) | T1SS (aprA, aprX, hasAp), T2SS (lasA, lasB, plcH, plcN, plcB, prpL, phoA, lipA, lipC, loxA, toxA, cbpD, paAP), T3SS (exoT, exoU, exoY), H2-T6SS (tssB2, pldA, pldB), H3-T6SS (tssB3), QS (lasI, lasR, rhlI, rhlR), others (fliC, rhlAB) |
a
Amino acid substitution compared to the sequences of Pseudomonas aeruginosa strain PAO1 (GenBank accession number AE004091).