FIG 1.

Randomized axelerated maximum likelihood (RAxML) tree of Blastocystis sp. based on small subunit rRNA (SSU rRNA) gene sequences. P. lacertae was chosen as an outgroup. Bootstrap values (1,000 replicates) are given as percentages above the individual branches. Branches with values of <50% are not shown. Blastocystis sp. isolated from schoolchildren’s feces (SF) are indicated in purple italics, participants’ feces (HF) in blue boldface, and pig feces (P) in red boldface, where the subtypes (STs) of humans are underlined and resident community number or Moo (M) is assigned after each fecal sample name. Animal isolates were labeled in parentheses after the accession number. Blue ◂, indicate Blastocystis isolated from open pig farm owners. The red dialog box indicates a major subtype with broad distribution across different communities.