Minimum spanning network inferred from SSU rRNA gene sequences of Blastocystis sp. in the communities (Moo 11, red; 18, green; 19, purple) at Thakradan Subdistrict, Chachoengsao Province, eastern Thailand. Each singleton is labeled using a sample code. Common haplotypes are presented in size with the number of Blastocystis isolates. Blastocystis sp. isolated from schoolchildren’s feces (SF) are indicated in purple italics, participants’ feces (HF) in blue boldface, and pig feces (P) in red boldface, where the subtypes (STs) of humans are underlined. The red dialog box indicates a major subtype with broad distribution across different communities.