TABLE 2.
List of recombinant proteins used in assay development
| Coronavirus species | Protein | Source | Catalog no. | Protein ID | Expression system | Purification tag | Coupling concn (μg per 500 μl) | Note |
|---|---|---|---|---|---|---|---|---|
| SARS-CoV-2 | N | AcroBiosystems | NUN-C5227 | AB_N | HEK293 cells | His | 5 | Final assay |
| N | RayBiotech | 230-30164-100 | RB_N | HEK293 cells | His | 5 | ||
| N | R&D Systems | 10474-CV | RD_N | Baculovirus-insect cells | His | 5 | Added to final assay subsequent to initial screening step | |
| N | Virogen | 00221-V | VR_N | E. coli | His | 25 | ||
| RBD | Acro Biosystems | SPD-C52H2 | AB_RBD | HEK293 cells | His | 5 | Final assay | |
| RBD | BEI Resources | NR-52306 | BEI_RBD | HEK293 cells | Hexa-his | 15 | EPA_RBD version in final assay | |
| RBD | Creative Diagnostics | DAGC089 | CD_RBD | HEK293 cells | Mouse IgG1 Fc | 5 | ||
| RBD | ProSci | 97-093 | PS_RBD | HEK293 cells | Mouse IgG Fc | 5 | ||
| RBD | R&D Systems | 10500-CV | RD_RBD | HEK293 cells | His | 15 | ||
| RBD | Sino Biological | 40592-V02H | SB_RBD | HEK293 cells | Human IgG1 Fc | 5 | Replaced with its His-tagged version (SB_RBDn) | |
| RBD | Sino Biological | 40592-V08H | SB_RBDn | HEK293 cells | His | 5 | Final assay | |
| S | AcroBiosystems | SPN-C52H8 | AB_S | HEK293 cells | His | 5 | Final assay | |
| S | BEI Resources | NR-52308 | BEI_S | Baculovirus-insect cells | Hexa-his | 15 | EPA_S version in final assay | |
| S | R&D Systems | 10549-CV | RD_S | HEK293 cells | His | 15 | Added to final assay subsequent to initial screening step | |
| S | Sino Biological | 40589-V08B1 | SB_S | Baculovirus-insect cells | His | 15 | ||
| S1 | BioVendor | RN0005100 | BV_S1 | HEK293 cells | Fc | 15 | ||
| S1 | Creative Diagnostics | DAGC091 | CD_S1 | HEK293 cells | His | 5 | Final assay | |
| S1 | GenScript | Z03501 | GS_S1 | Human cells | Tag-free | 5 | ||
| S1 | NativeAntigen | REC31806 | NA_S1 | HEK293 cells | Sheep IgG Fc | 15 | ||
| S1 | Sino Biological | 40591-V08H | SB_S1 | HEK293 cells | His | 5 | Selected for final assay, but later removed | |
| S1 mosaic | Virogen | 00220-V | CR_S1 | E. coli | His | 15 | ||
| S2 | BioVendor | RN0004100 | BV_S2 | HEK293 cells | IgG Fc | 15 | ||
| S2 | Native Antigen | REC31807 | NA_S2 | HEK293 cells | Sheep IgG Fc | 25 | ||
| S2 | ProSci | 97-079 | PS_S2 | HEK293 cells | His | 5 | Replaced with PS_S2i version | |
| S2 | ProSci | 10-115 | PS_S2i | Baculovirus-insect cells | His | 5 | Final assay | |
| α-CoV 229E | S | Sino Biological | 40605-V08B | SB_229E_S | Baculovirus-insect cells | His | 15 | Final assay |
| S1 | AcroBiosystems | SIN-V52H4 | AB_229E_S1 | HEK293 cells | His | 15 | ||
| S1 | Sino Biological | 40601-V08H | SB_229E_S1 | HEK293 cells | His | 5 | ||
| α-CoV NL63 | S | Sino Biological | 40604-V08B | SB_NL63_S | Baculovirus-insect cells | His | 15 | Final assay |
| S1 | AcroBiosystems | SIN-V52H3 | AB_NL63_S1 | HEK293 cells | His | 15 | ||
| S1 | Sino Biological | 40600-V08H | SB_NL63_S1 | HEK293 cells | His | 5 | ||
| β-CoV HKU1 | S | Sino Biological | 40606-V08B | SB_HKU1_S | Baculovirus-insect cells | His | 15 | Final assay |
| S1 | AcroBiosystems | SIN-V52H6 | AB_HKU1_S1 | HEK293 cells | His | 15 | ||
| S1 | Sino Biological | 40602-V08H | SB_HKU1_S1 | HEK293 cells | His | 5 | ||
| β-CoV OC43 | S | Sino Biological | 40607-V08B | SB_OC43_S | Baculovirus-insect cells | His | 15 | Final assay |
| S1 | AcroBiosystems | SIN-V52H5 | AB_OC43_S1 | HEK293 cells | His | 5 | ||