Table 1.

Base sequences of forward/reverse primers, capture/detection DNA probes, and bridge DNAs for E and RdRp genes used in AuNDS-based SERS-PCR assays.

Target genes and Probe DNAs	Base sequence (5'- 3')	Fragment size (bp)
E gene	NC_004718 (26141-26269)	128
Forward primer	ACAGGTACGTTAATAGTTAATAGCGT	26
Reverse primer	ATATTGCAGCAGTACGCACACA	22
Capture DNA probe	GATGGCTAGTGTTTTTTTTT-(CH2)3-SH	20
Detection DNA probe	SH-(CH2)6-TTTTTTTTCGAAGCGCAGTA	20
Bridge DNA	iSP3-ACACTAGCCATCCTTACTGCGCTTCG-iSP3	26
RdRp gene	MN908947 (15431-15531)	100
Forward primer	GTGARATGGTCATGTGTGGCGG	22
Reverse primer	CARATGTTAAASACACTATTAGCATA	26
Capture DNA probe	AGGTTCCACCTGTTTTTTTT-(CH2)3-SH	20
Detection DNA probe	SH-(CH2)6-TTTTTTTTGCATCTCCTGAT	20
Bridge DNA	iSP3-CAGGTGGAACCTCATCAGGAGATGC-iSP3	25

*R = A/G, S = C/G. iSP₃ = -(CH₂)₂CH₃, NC_004718 and MN908947 = GenBank database.