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. 2021 Sep 8;37(7):386–393. doi: 10.1089/jop.2020.0119

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© Eva Hernandez et al. 2021; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 4. — Autophagy is required for protective effect of artificial tears against desiccation. WT and autophagy-deficient (ATG4B C74A) cells were overnight exposed to artificial tears before desiccation. Cell viability was evaluated by MTT method. Histograms represent numbers of surviving cells after desiccation (in fold changes compared with untreated control cells). Results indicate mean ± SD of 3 independent experiments. ***Statistically significant difference compared with the untreated control cells, P < 0.001 (one-way ANOVA test). ANOVA, analysis of variance.