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Relative mRNA levels of X-linked genes Kdm6a, Eif2s3x, Ddx3x, Kdm5c, Mecp2, Usp9x, and Syp in FCG hypothalamic neurons at 3 DIV. Kdm6a, Eif2s3x, and Ddx3x show higher expression levels in XX compared to XY cultures, regardless of gonadal sex (XXf and XXm > XYf and XYm). Data are mean ± SEM. n = 5–7 independent cultures for each genotype. *p < 0.05; **p < 0.01
