Fig. 1.
Reduced menin expression leads to a decrease in ERα expression. a Co-immunofluorescence against menin and ERα on mammary gland sections from Men1F/FWapCre− mice (upper panel) and Men1F/FWapCre+ mice (lower panel) at < 12 months of age. Quantification of IF signals for menin and ERα is shown on the right. b Western blot analyses using antibodies against menin and ERα in MCF7, T47D, and ZR75-1 cells treated with siRNA control (siCtrl) or siRNA targeting the MEN1 gene (siMEN1 hs1). c Quantitative RT-qPCR analyses of MEN1 and ESR1 expression in MCF7 and T47D cells treated with siCtrl or two different siMEN1 (hs1 or hs2). d Western blot analyses using antibodies against menin and ERα in MCF7 and T47D treated with siRNA control (siCtrl) or siMEN1 hs1 and then subjected to estradiol (E2) stimulation at a concentration of 10 nM. e Quantitative RT-qPCR analyses of the MEN1 and ESR1 expression in MCF7 and T47D cells treated with siCtrl or siMEN1 hs1 and then subjected to estradiol (E2) stimulation at a concentration of 10 nM. PS2 transcript was used as a positive control. All data are expressed as mean ± SEM, ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001