Fig. 2.

Recruitment and effects of menin on the proximal ESR1 promoter. a Schematic diagram of the different regions of the ESR1 promoter studied, including A, B, and C regions of ESR1, that were cloned into luciferase reporter constructs (see c). b ChIP-Quantitative PCR analyses of menin binding on the − 2500 bp/ + 2000 bp area flanking the transcription start site (TSS) of ESR1 in MCF7 (left) and T47D (right) cells. c MCF7 cells were transfected with siCtrl or siMEN1 hs1 and pGL3-PrAB or pGL3-PrC luciferase reporter. After 48 h of transfection, luciferase activities were measured in triplicate for each condition. All the data are expressed as mean ± SEM, ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001