TABLE 2.
Number of chlamydial inclusions recovered from spiked specimens (eight wells) after 7 days of incubation
| Specimen type and no. | Median (range) no. of IFU recovered
|
Pa | |
|---|---|---|---|
| Standard method | PEG pretreatment | ||
| NP | |||
| No additional centrifugations | |||
| 1 | 64.5 (24–93) | 37.5 (24–59) | 0.0513 |
| 2 | 148.5 (115–221) | 141.5 (87–189) | 0.8335 |
| 3 | 118 (96–150) | 110 (30–197) | 0.7525 |
| 4 | 87.5 (57–126) | 74.5 (50–133) | 0.9163 |
| Additional centrifugations | |||
| 1 | >500 (>500) | >500 (>500) | NDb |
| 2 | >500 (>500) | >500 (>500) | ND |
| 3 | >500 (>500) | >500 (>500) | ND |
| 4 | >500 (>500) | >500 (>500) | ND |
| Blood | |||
| No additional centrifugations | |||
| 1 | 4.5 (0–6) | 4.5 (1–8) | 0.9571 |
| 2 | 4.5 (1–7) | 6 (0–12) | 0.2911 |
| 3 | 16 (4–23) | 11 (5–16) | 0.1242 |
| 4 | 7 (1–21) | 7 (0–26) | 0.4929 |
| Additional centrifugations | |||
| 1 | 164 (80–>300) | 235 (100–>300) | 0.3270 |
| 2 | >500 (>500) | >500 (>500) | ND |
| 3 | >500 (>500) | >500 (>500) | ND |
| 4 | >500 (>500) | >500 (>500) | ND |
a
Statistical analysis was performed with the Wilcoxon rank sum test, with P ≤ 0.05 considered significant.
b
ND, not determined.