Table 4.
Brief compilation regarding the application of NGS techniques in grass pea.
| S No. | Genotype used | Trait | Sequencing platform | Contigs | Transcripts | Unigenes | No. of primers detected | No. of genes annotated | References |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Eight grass pea (L. sativus) accessions consisted of two Chinese, two Asian, one African and three European accessions | - | Roche 454 GS FLX Titanium platform | 370,079 (453 bp) | - | - | 651,827 | - | Yang et al., 2014 |
| 2 | BGE015746 and BGE024709 | Rust resistance | Illumina Hiseq 2000 | 134,914 | - | 2,634 SNPs 200 EST-SSR | 50,937 (60.4% into functional categories) | Almeida et al., 2014b | |
| 3 | BGE015746 | Aschochyta blight resistance | Illumina Genome Analyser IIx | - | - | 14,386 | - | 13,773 | Almeida et al., 2015 |
| 4 | Rewa-2 | - | Illumina HiSeq 2500 | 15,779,854 | 49,280 | 33,042 | 1139 SSRs | - | Chapman, 2015 |
| 5 | RQ23 and RQ36 | - | Illumina NextSeq 500 | 1,970,104 | 142,053 | 27,431 | 3,204 EST-SSR, 146,406 SNP | - | Hao et al., 2017 |
| 6 | LZ | β-ODAP | Illumina HiSeq 3000 | 287,695 | 213,258 | 213,258 | - | 27,032 | Xu et al., 2018 |
| 7 | LP-24 | Drought | Illumina HiSeq 2500 | - | 165,910 | 41,661 | 8079 SSRs | 31,368 | Rathi et al., 2019 |