Skip to main content
. 2021 Nov 1;8:239. doi: 10.1038/s41438-021-00669-x

Fig. 2. Female germline development in pineapple.

Fig. 2

A–G DIC observation of the ovule primordium at the AC (A), MMC (B), meiosis (C), and FM (D) stages; developing ovules at the two-nuclear (E) and four-nuclear stages of megagametogenesis (F); and a fully differentiated ovule containing a cellularized female gametophyte (G). H–M Semithin section of ovules at the MMC (H), dyad (I), tetrad (J), and FM (K) stages and fully differentiated ovules containing two SNs (L) and a CN (M). N–S Cell plate formation during megasporogenesis as revealed by callose staining. T Whole-mount immunolocalization analysis of an ovule in Arabidopsis using a DMC1 antibody. U–V. Whole-mount immunolocalization analysis of an ovule in pineapple using a DMC1 antibody. Green represents the DMC1 signal. AC archesporial cell, CN central cell nucleus, DM degenerated megaspore, EN egg cell nucleus, FM functional megaspore, MMC megaspore mother cell, SC synergid cell, SN synergid cell nucleus. A–G, Bars = 50 μm, H–M, Bars = 20 μm, N–S, Bars = 50 μm, T–V, Bars = 10 μm