FIGURE 4.

Effects of anti-hypertensive drugs on ponatinib or HQP1351 induced dorsal aorta constriction. (A) Embryos were co-treated with the indicated compounds from 2 to 4 dpf and analyzed as in Figure 1B. Valsartan and azilsartan are ARBs and able to rescue ponatinib-induced dorsal aorta constriction. Other anti-hypertensive drugs are less efficacious to minimize ponatinib-induced vascular defects. (B) Valsartan, azilsartan, benazepril and metolazone can rescue HQP1351-induced vascular constriction while macitentan, captopril, acebutolol and amlodipine cannot. Assays were repeated and analyzed as described in Figure 1B. Red arrow: dorsal aorta. Scale bar: 50 µm.