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. 2021 Oct 18;12:738559. doi: 10.3389/fgene.2021.738559

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Copyright © 2021 Yufeng, Ming and Dandan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ectopic expression of miR-320d inhibits the proliferation, invasion, and migration of EGFR-positive CRC cells. (A) MTT cell proliferation assays in EGFR-positive CRC cells transfected with indicated miRNAs. (B) Representative photomicrographs of wound-healing assay in EGFR-positive CRC cells after transfection of miRNAs. (C) Transwell assays of EGFR-positive CRC cells carrying different miRNAs. The total number of cells in the field was counted manually. Assays were performed in triplicates. Mean ± SD is shown. Statistical analysis was conducted using one-way ANOVA. **p< 0.01. (D) Western blots of E-cadherin and vimentin in EGFR-positive CRC cells transfected with different miRNAs.; Abbreviations: MTT, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; NC, negative control.