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. 2021 Oct 18;12:738559. doi: 10.3389/fgene.2021.738559

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Copyright © 2021 Yufeng, Ming and Dandan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-320d targets the 3′-UTR of TUSC3 mRNA. (A) qRT-PCR analysis of TUSC3 mRNA expression in EGFR-positive CRC cells transfected with the indicated miRNAs. (B) Western blots of TUSC3 in EGFR-positive CRC cells transfected with different miRNAs. (C) Luciferase activity of reporter plasmids carrying wild-type or mutant TUSC3 3′-UTR in EGFR-positive CRC cells co-transfected with the indicated miRNAs. Assays were performed in triplicates. Mean ± SD is shown. Statistical analysis was conducted using Student’s t test. *p < 0.05. **p < 0.01.