Table 1.
Summary of amplitudes and phases estimated from nonlinear sine function fits to the HR measurements under the indicated experimental conditions.
| Condition | N | Absolute HR | Normalized HR | |||
|---|---|---|---|---|---|---|
| Mean HR (BPM) | Amplitude | Phase (radians) | Amplitude | Phase (radians) | ||
| Anesthetized Mice | ||||||
| Baseline | 13 | 544±65 | 47.1±7.4 | 2.91±0.12 | 0.09±0.02 | 2.91±0.17 |
| SNA Block | 7 | 424±36*** | 29.1±4.4* | 2.59±0.14 | 0.07±0.01n.s | 2.64±0.19 |
| PNA Block | 6 | 592±46*** | 16.4±9.4* | 2.59±0.46 | 0.02±0.02* | 2.43±0.68 |
| ANS Block | 6 | 485±36*** | 11.9±5.9** | 1.41±0.79 | 0.03±0.01** | 1.42±0.77 |
| Conscious Mice | ||||||
| SED | 5 | 568±54 | 65.9±13.3 | 2.84±0.18 | 0.12±0.03* | 2.84±0.24 |
| WHEEL | 5 | 577±91 | 122.5±11.6** | 2.45±0.09 | 0.18±0.03* | 2.33±0.17 |
The amplitudes and phase shifts were obtained by nonlinear fits of the HR results to sine functions for various conditions: Baseline = no blockers, SNA Block = with propranolol, PNA Block = with atropine, ANS Block = with atropine and propranolol, SED = conscious mice with no wheel access, and WHEEL = conscious mice with wheel access. Data presented as Mean ± S.D.
*
p<0.05
**
p<0.01
***
p<0.001, and n.s(P=0.06) compared to baseline or sedentary mice with no wheel access using an extra sum-of-squares F test. Additional within- and between-group comparisons are outlined in the Results.