FIG. 6.

In vivo regulation of 5′ splice site selection by SRrp86. HeLa cells were transfected with the in vivo splicing construct pcDNA 5′D-16X (A) or adenovirus E1A (B). Cells were cotransfected with either a control vector (pcDNA) or a vector expressing SRrp86, the ΔRS mutant, or ASF/SF2, as indicated. RNA was isolated 48 h after transfection, and RT-PCR was performed to amplify the three possible spliced products, which were quantitated by PhosphorImager analysis, as indicated. The positions of the primers used for RT-PCR are indicated by the arrows.