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. 2021 Oct 18;9:739944. doi: 10.3389/fcell.2021.739944

FIGURE 1.

FIGURE 1

WW domain-containing E3 ubiquitin protein ligase 1 expression changes in the hearts of mice and rhesus monkeys after simulated microgravity. (A) Representative western blotting analysis of WWP1 expression in cardiac extracts of adult mice following tail suspension (TS) for 6 weeks (n = 3 for each group). (B) Quantification of WWP1 protein levels of (A). (C) Immunohistochemistry for WWP1 (brown) in paraffin section from mouse hearts at 6 weeks after tail suspension. Scale bars, 200 μm. (D) Representative western blotting analysis of WWP1 expression in cardiac extracts of adult rhesus monkeys following bed rest (BR) for 6 weeks (Ctrl, n = 3; BR, n = 5). (E) Quantification of WWP1 protein levels of (D). (F) Immunohistochemistry for WWP1 (brown) in paraffin section from rhesus monkey hearts at 6 weeks after bed rest. Scale bars, 200 μm. Data represent the means ± SEM. *P < 0.05, **P < 0.01. WWP1, WW domain-containing E3 ubiquitin protein ligase 1; TS, tail suspension; BR, bed rest.