Table 1.
Inter and Intra Assay Reproducibility of Genotypic Analysis
| Target Gene | Intra-Assay | Inter-Assay | ||||
|---|---|---|---|---|---|---|
| Mean (Ct) | SD | CV% | Mean (Ct) | SD | CV% | |
| MN908947.3-Wuhan-hu-1 | ||||||
| L452R-WILD | 25.16 | 0.29 | 1.17 | 23.79 | 1.26 | 5.28 |
| E484K-WILD | 22.86 | 0.09 | 0.4 | 23.08 | 0.21 | 0.92 |
| D614G-WILD | 23.93 | 0.35 | 1.48 | 23.62 | 0.16 | 0.68 |
| delH69V70-WILD | 22.6 | 0.15 | 0.66 | 23.01 | 0.12 | 0.52 |
| A701V-WILD | 22.89 | 0.12 | 0.52 | 22.48 | 0.3 | 1.33 |
| K417T-WILD | 24.1 | 0.17 | 0.71 | 23.95 | 0.41 | 1.71 |
| K417N-WILD | 24.29 | 0.12 | 0.48 | 24.05 | 0.2 | 0.82 |
| N501Y-WILD | 22.55 | 0.16 | 0.71 | 22.65 | 0.2 | 0.88 |
| Q27stop-WILD | 22.97 | 0.19 | 0.84 | 23.28 | 0.46 | 1.97 |
| L242_244L-WILD | 24.39 | 0.13 | 0.54 | 23.76 | 0.7 | 2.94 |
| P681R-WILD | 23.41 | 0.01 | 0.05 | 23.4 | 0.06 | 0.24 |
| T20N-WILD | 24.57 | 0.19 | 0.77 | 24.71 | 0.49 | 1.99 |
| B. 1.1.7_601443 | ||||||
| D614G-MUTANT | 24.17 | 0.02 | 0.08 | 24.22 | 0.13 | 0.55 |
| delH69V70- MUTANT | 22.76 | 0.02 | 0.1 | 22.8 | 0.08 | 0.35 |
| N501Y- MUTANT | 22.78 | 0.03 | 0.11 | 22.89 | 0.23 | 0.99 |
| Q27stop- MUTANT | 24.31 | 0.04 | 0.16 | 24.81 | 0.28 | 1.14 |
| B.1.351_678597 | ||||||
| D614G-MUTANT | 24.613 | 0.015 | 0.06 | 24.59 | 0.03 | 0.13 |
| E484K- MUTANT | 23.773 | 0.028 | 0.12 | 24.13 | 0.22 | 0.89 |
| N501Y- MUTANT | 23.112 | 0.041 | 0.178 | 23.13 | 0.22 | 0.97 |
| K417N- MUTANT | 24.686 | 0.081 | 0.33 | 24.67 | 0.16 | 0.66 |
| A701V- MUTANT | 23.96 | 0.089 | 0.37 | 23.96 | 0.22 | 0.9 |
| L242_244L –MUTANT | 25.792 | 0.017 | 0.066 | 25.996 | 0.552 | 2.122 |
| P.1_792683 | ||||||
| D614G-MUTANT | 24.19 | 0.03 | 0.14 | 23.99 | 0.12 | 0.52 |
| E484K- MUTANT | 23.24 | 0.02 | 0.1 | 23.49 | 0.17 | 0.7 |
| N501Y- MUTANT | 22.63 | 0.01 | 0.02 | 22.54 | 0.24 | 1.07 |
| K417T- MUTANT | 23.82 | 0.06 | 0.24 | 23.65 | 0.16 | 0.67 |
| T20N- MUTANT | 24.61 | 0.06 | 0.25 | 24.88 | 0.34 | 1.38 |
| B.1.351 (South Africa/KRISP-K005325/2020) | ||||||
| D614G-MUTANT | 18.23 | 0.09 | 0 | 18.12 | 0.09 | 0 |
| E484K-MUTANT | 17.95 | 0.02 | 0 | 17.45 | 0.33 | 0.02 |
| N501Y-MUTANT | 16.48 | 0.06 | 0 | 16.45 | 0.18 | 0.01 |
| K417N-MUTANT | 18.01 | 0.11 | 0.01 | 17.96 | 0.39 | 0.02 |
| A701V-MUTANT | 17.48 | 0.06 | 0 | 17.61 | 0.13 | 0.01 |
| L242_244L-MUTANT | 19.33 | 0.03 | 0 | 19.23 | 0.07 | 0 |
| B.1.1.7 (England/204820464/2020) | ||||||
| D614G-MUTANT | 18.674 | 0.062 | 0.003 | 18.608 | 0.054 | 0.003 |
| delH69V70-MUTANT | 17.853 | 0.08 | 0.004 | 17.55 | 0.256 | 0.015 |
| N501Y-MUTANT | 16.827 | 0.075 | 0.004 | 16.874 | 0.089 | 0.005 |
| Q27stop-MUTANT | 18.477 | 0.058 | 0.003 | 18.425 | 0.125 | 0.007 |
| B.1.617.1_1662307 | ||||||
| L452R-MUTANT | 25.24 | 0.07 | 0.28 | 24.09 | 0.739 | 3.07 |
| D614G-MUTANT | 24.7 | 0.01 | 0.05 | 25.39 | 0.382 | 1.5 |
| E484Q- MUTANT | 24.22 | 0.015 | 0.06 | 24.26 | 0.36 | 1.49 |
| P681R-MUTANT | 24.38 | 0.03 | 0.13 | 24.85 | 0.361 | 1.45 |