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. 2021 Oct 18;9:724282. doi: 10.3389/fcell.2021.724282

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Copyright © 2021 Huang, Yang, Zhang, Liu, Fei, Tong, Niu and Liang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In situ protein expression analysis of eight m⁶A key regulators in pancreatic ductal adenocarcinoma (PDAC). (A) Representative immuno-histochemical staining showing the expressions of METTL3, METTL14, WTAP, FTO, ALKBH5, and YTHDF1-3 in normal pancreatic ductal epithelial cells (red arrows) in para-tumor samples, tumor cells (blue arrows), and stromal cells (black arrows) in tumor samples. Enlarged images in the box are shown in the upper right. (B) Scatter plots showing the H-scores of each gene in para-tumor, tumor and stroma cells are shown in the right panel. Case numbers are shown in brackets. Scale bars, 100 or 25 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.