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. 2021 Oct 18;9:724282. doi: 10.3389/fcell.2021.724282

FIGURE 7.

FIGURE 7

ALKBH5 protects against tumor progression in PDAC by targeting regulators of iron metabolism. (A) Western blot analysis showing the expressions of IRP2, SNAI1, E-cadherin, and N-cadherin in ALKBH5-overexpressing cells. EV, empty vector; OE, ALKBH5-overexpressing cells. GAPDH is used as an internal control. (B) Intracellular iron assay showing the effect of ALKBH5 overexpression on intracellular iron levels. (C) Western blot analysis showing the effect of FBXL5 knockdown in rescuing the expression of IRP2, SNAI1, E-cadherin, and N-cadherin in ALKBH5-overexpressing cells. SC, scramble control; KD-1, FBXL5 knockdown-1; KD-2, FBXL5 knockdown-2. (D) Intracellular iron assay showing the effect of FBXL5 knockdown in restoring intracellular iron levels in ALBKH5-overexpressing cells. (E) Cell migration and invasion assays of FBXL5 knockdown in OE. (F) Statistical analysis for the result of transwell assays in (E). Cell numbers per field were presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (G) Schematic diagram depicting the role of ALKBH5 in attenuating pancreatic tumorigenesis through targeting regulators of iron metabolism. (Created with BioRender.com).