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. 2021 Oct 18;12:757215. doi: 10.3389/fmicb.2021.757215

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Copyright © 2021 Hansen, Maschkowitz, Podschun and Fickenscher.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analyses of COLO-205 cell lysates treated with different batches of IL-26 dialysates, positive control, and negative control. Top: Detection of phospho-STAT3 using primary polyclonal rabbit-raised phospho-STAT3 antibodies. Bottom: Detection of STAT3α and STAT3β using primary polyclonal rabbit-raised STAT3 antibodies. For both analyses, horseradish peroxidase-conjugated anti-rabbit IgG antibodies were used as second reagents. Chemiluminescence detection was performed with a charge-coupled device camera (LAS-3000, Fujifilm, Japan).