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. 2021 Oct 18;9:731015. doi: 10.3389/fcell.2021.731015

FIGURE 8.

FIGURE 8

Mitochondrial function and markers of oxidative stress in control and AMPKα1α2-KO mice. (A–H) Function of cardiac mitochondria isolated 10 weeks after tamoxifen administration. State 3 and 4 respiration with glutamate and malate (A), succinate in presence of rotenone (B), palmitoylcarnitine (C) and DNP-uncoupled respiration with succinate as substrate (D). Activity of mitochondrial enzymes: complex I (E), complex IV (F), carnitine palmitoyltransferase (G). Calcium retention capacity (CRC) in presence and absence of cyclosporine A, CsA (H). (I,J) Markers of oxidative stress 52 weeks after tamoxifen administration: TBARS (I) and THIOLS (J). Control (white bar), KO (gray bar). Data are presented as means ± SEM; n = 5–13 (A–G), 4–5 (H), 10–13 (I), 5 (J). For (H) statistical analysis was done by 2-way Repeated Measure ANOVA; the resulting significance values are given for two main effects of genotype PGen and presence of cyclosporine A PCsA, and their interaction PGen × CsA. Other comparisons were performed either using t-test or in (A–C,E) Mann–Whitney test.