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. 2021 Nov 1;22:535. doi: 10.1186/s12859-021-04455-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Overview of the FASTAFS file format specification. (top) A flowchart of the fastafs cache procedure. (bottom) The layout of the FASTAFS format consists of four blocks, starting with the file header, followed by the per-sequence data, the per-sequence header data and a metadata block. The file header has a file pointer to the per-sequence header block, where each sequence has a file pointer to its data. The file ends with a metadata block, currently supporting a CRC32 checksum. The raw FASTAFS file is subsequently compressed with zstd-seekable. The full specification is available on the website: https://github.com/yhoogstrate/fastafs/blob/master/doc/FASTAFS-FORMAT-SPECIFICATION.md