Figure 5.

KRAS-mutant tumor cells might transform into stem-like cells. Panc1 cells were cultured for 3 days in the presence of 5 or 25 µM of dextrose. (A) The expression level of OCT4, BMI1, NANOG, SOX2, CD24, E-cadherin, β-catenin, and vimentin was detected by RT-PCR. Data are presented as mean ± SEM of three independent experiments. (B) Vimentin expression was analyzed by immunofluorescent staining (blue, nucleus; green, vimentin). Original magnification ×600. *p < 0.05, **p < 0.005, ***p < 0.0005.