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. 2021 Oct 17;12(23):7052–7068. doi: 10.7150/jca.64638

Figure 3.

Figure 3

Effects of H. zeylanica-E2 on apoptosis of GC cells through changes in caspase 3/7 activity. (A) AGS cells were treated with H. zeylanica-E2 (10 μg/mL), and (B) BGC823 cells were treated with H. zeylanica-E2 (5 μg/mL) for 0, 24, or 48 h. The cells from different experimental groups were collected and incubated with Muse™ Caspase-3/7 working solution (Merck) for 30 min at 37 °C and then mixed thoroughly with 7-AAD while protected from light at room temperature for 5 min and analyzed using a Muse™ Cell Analyzer (Merck). Four populations of cells (live, necrotic, apoptotic, and late apoptotic/dead) were quantified. The lower left shows live cells: caspase-3/7- and 7-AAD-; lower right shows apoptotic cells exhibiting caspase-3/7 activity: caspase-3/7+ and 7-AAD-; upper left shows necrotic cells: caspase-3/7- and 7-AAD+; and upper right shows late apoptotic/dead cells: caspase-3/7+ and 7-AAD+. Data are presented as mean ± SEM of at least three independent experiments. Student's-t-test or one-way ANOVA was used for comparison with the control. (**p < 0.01, *p < 0.05).