Figure 4.

Effects of H. zeylanica-E2 on apoptosis of GC cells through changes in Bcl-2, Bax, and cleaved PARP. (A) AGS and (B) BGC823 cells were treated without or with H. zeylanica-E2 for 0, 24, or 48 h. The contents of proteins related to apoptosis (cleaved PARP, Bcl-2, and Bax) were quantified by Western blotting. GAPDH was used as the internal control, and the expression ratio of these proteins was quantified. Data are presented as mean ± SEM of three independent experiments. Student's-t-test or one-way ANOVA was used for comparison with the control. (*p < 0.05, **p < 0.01).