Figure 1.
Experimental design for the evaluation of oxygen modulation on hMSC secretome. SFCM from hMSC cultures were collected under specified oxygen tensions and used for subsequent experimentation. The 21% O2 incubator providing a AO environment shows no fluctuation of gas phase oxygen at the cellular level during standard incubation or processing the cells outside of the incubator. In contrast, 2% O2 incubators provide an IH environment with fluctuations in oxygen concentration when processing cultures outside of the incubator during seeding, changing media, passaging, door opening and closing. The 2% O2 Workstation provides a P environment characterized by steady state oxygen levels in the closed culture system. Solid lines indicate the oxygen levels present in the specific culture condition.